Success and integrated amplitude of motor responses evoked by short-pulse PS of VGluT2 GRN neurons in the resting mouse.

(A) Left, examples of rectified averaged EMG response of the iTA to photostimulation. Distribution of EMG responses (middle) from a single animal photostimulated at threshold (4.8 mW). Right, failures are in gray, successes in black. (B) Individual traces of the example shown in (A). The color of traces matches the histogram in (A). The response time window corresponds to the period delimited in (A) by the two arrows. (C) Boxplot of success rate (in %) of motor responses to PS at and above threshold. Wilcoxon signed rank test: *p-value S1 Data. a.u., arbitrary units; cGL, contralateral gastrocnemius lateralis; cTA, contralateral tibialis anterior; EMG, electromyographic; GRN, gigantocellular reticular nucleus; iGL, ipsilateral gastrocnemius lateralis; iTA, ipsilateral tibialis anterior; PS, photostimulation; VGluT2, vesicular glutamate transporter 2.