Separation of bulge and SHG GFP-expressing melanocyte precursor cells of telogen HFs.

(A) Experimental scheme: McSCs identified in HF bulge and SHG of Dct-H2BGFP mice were separated using FACS with GFP and anti-CD34. (B) Separation of bulge (CD34+GFP+) and SHG (CD34-GFP+) melanocyte precursor cells and CD34+GFP- and CD34-GFP- dermal cells using FACS with GFP and anti-CD34, showing a representative image of the FACS. DP = Double positive, SP = Single positive and DN = Double negative. (C) Quantitative RT-PCR analysis for the expression of Dct, Krt14, Cdh3, and Cd34 genes among the CD34+GFP+(bulge), CD34-GFP+(SHG), CD34+GFP- and CD34-GFP- sorted cell populations. Here, Gapdh was used as reference gene, (*P ≤ 0.01 by ANOVA). (D) Quantitative RT-PCR analysis for the expression of Tyr, Tyrp1 and Pmel17 in bulge (CD34+GFP+) and SHG (CD34+GFP+) sorted cells. Here, Gapdh was used as reference gene. (*P ≤ 0.01 by ANOVA).