Development of functional human CD34⁺ HPCs in BM of humanized mice.

<p>(A) Representative dot plots showing distribution of human early CD34⁺CD38^- and intermediate CD34⁺CD38⁺ HPCs in BM of humanized mice. Numbers indicate percentages of CD34⁺CD38^- and CD34⁺CD38⁺ HPCs (gated on hCD45⁺mCD45^-VLD^-Lin^-CD34⁺ cells). Data are representative of two independent experiments with mice reconstituted with three human donors. (B) Pooled data indicating numbers of colonies that developed from human CD34⁺ HPCs from BM of humanized mice and human fetal livers (n = 6). Total colonies, CFU-GM, colony-forming unit-granulocyte, macrophage; CFU-E, colony-forming unit-erythroid; CFU-GEMM, colony-forming unit-granulocyte, erythroid, macrophage, megakaryocyte. (C) Representative histograms showing proliferation of CD34⁺ HPCs <i>in vivo</i> through BrdU labeling in humanized mice. PBS mouse samples were used as controls (upper panels). Numbers indicate percentages of BrdU⁺ HPCs within CD34⁺ HPC subsets. (D) Pooled data indicate percentages of BrdU⁺ CD34⁺ HPC subsets in BM of humanized mice (n = 5). (B, D) Data are shown as the mean ± s.e.m.