Pro-apoptotic changes in ARPE-19 cells.

(A) Bax and Bcl-2 protein expression in ARPE-19 under the pro-oxidants conditions treated for 24 h was assessed by western blot. (B) Densitogram analysis of Bax/Bcl-2 ratio normalized to control. (C) pNF-κB and NF-κB protein expression in ARPE-19 under the pro-oxidants conditions treated for 24 h was assessed by western blot. (D) Densitogram analysis of pNF-κB/NF-κB ratio normalized to control. (E) NFE2L2 gene expression was quantified by qPCR in ARPE-19 cells exposed to pro-oxidant conditions for 24 h. Values are expressed as Mean ± SEM, n = 3. *;#p .05, **,##p .01, ***p .001, considered as significant. *Control vs pro-oxidants, #H2O2 vs NAC.