Effect of Tβ4 on pro- and anti-apoptotic genes under oxidative stress in cardiac fibroblasts.

<p>Cells were treated with H₂O₂ in the presence and absence of Tβ4 and (<b>A</b>) Caspase-3, (<b>B</b>) Bax and (<b>C</b>) Bcl₂ mRNA expression were analyzed at 12 h and 24 h, respectively by qRT-PCR. Data represent the means±SEM of at least three separate experiments. (<b>D</b>) Protein expression of Caspase-3, Bax and Bcl₂ at 12 h and 24 h, respectively. GAPDH was used as loading control for the experiment. (<b>E</b>) GAPDH was used as internal loading control for the experiment. (<b>E</b>) Graph shows the relative fold change in the protein expression of Bax, Bcl₂ and caspase-3, respectively by densitometry. Data represent means±SEM from 3 individual experiments. * denotes p<0.05 compared to controls while^# denotes p<0.05, compared to the H₂O₂-treated group and ^@ means p = ns compared, to the H₂O₂-treated group.