gypsy-CLEVR activity in glial cells is increased with age.

(A) Optical sections are shown from anterior brain regions from 2-day-, 7-day-, and 30-day-old gypsy-CLEVR transgenic flies without and with a gypsy-IR transgene. Glial nuclei are independently labeled with antibodies against the glial Repo marker (cyan). Neuronal nuclei are labeled with antibodies against the Elav marker (blue), and gypsy-CLEVR replication is labeled with mCherry (red). High-magnification images (right panels) from within the regions marked with yellow dashed box (left panels) are shown for each experimental group. Scale bar = 20 μm. (B) Numbers of mCherry-labeled glial cells (mean ± SEM) within anterior brain sections are shown for each experimental group (repo>LacZ+gypsy-CLEVR, D2: 32.1 ± 2.7, n = 8; D7: 118.8 ± 18.1, n = 8; D30: 404.6 ± 16.4, n = 10; repo>gypsy-IR+gypsy-CLEVR, D2: 21.1 ± 3.5, n = 7; D7: 54.4 ± 5.9, n = 11; D30: 189.6 ± 10.6, n = 12) (see S4 Data) (*p p p p t test). CLEVR, cellular labeling of endogenous retrovirus replication; D, day; IR, inverted repeat.