hHtt103Q expression in glial cell lineage induces pathological change in the central nervous system.

<p>(A) In <i>repo; hHtt103Q</i> flies, mutant Htt stained by anti-hHtt N-18 antibody was clearly colocalized with a glial cell marker, repo stained by anti-repo antibody (upper panels, arrow heads). High magnification shows a negative relationship between the expression level of hHtt103Q and that of repo (middle panels, dotted squares numbered). Glial cells marked with squares in middle panels are magnified in lower panels. Four examples of high-Htt103Q cells (1∼4) and 4 examples of high-repo cells (5∼8) are shown. (B) Morphological changes of brains in the <i>gcm; hHtt103Q</i> and <i>repo; hHtt103Q</i> flies. The coronal sections of the head were stained with toluidine blue, anti-repo antibody (glial cell marker), and anti-elav antibody (pan-neuron marker). Arrows in <i>repo; hHtt103Q</i> flies indicate vacuolar changes.