Additional file 4: of Placental extract suppresses differentiation of 3T3-L1 preadipocytes to mature adipocytes via accelerated activation of p38 MAPK during the early phase of adipogenesis

Figure S4. 3T3-L1 cells which reached confluence were cultured on a 12-well plate with IDM in the presence of PPE (PPE/IDM 1 h), fraction No. 9 (Fraction No. 9/IDM 1 h) or PBS (Vehicle/IDM 1 h) for 24 h. Cells were then lysed in 1 × SDS sample buffer, and the cell lysates were subjected to western blotting analysis with antibodies against phospho-p38 MAPK, total p38 MAPK or β-Actin. The relative intensity of each band of the phosphorylated forms after normalization for the levels in the total forms and β-Actin is shown as a bar graph. The experiments were performed in triplicate, and the data are presented as the mean ± SEM (n = 3). **p < 0.01 vs. Vehicle/IDM 1 h. Experiments were repeated at least three times, and representative results are shown. (TIF 1742 kb