Additional file 1: of Fast Adipogenesis Tracking System (FATS)—a robust, high-throughput, automation-ready adipogenesis quantification technique

Figure S1. Correlation of adipogenic scores and adipogenic genes expression in 3T3-L1 cells. Figure S2. Sizes per lipid droplet from Nile Red staining were estimated with the modified method using background subtraction and circular Hough transform during adipogenesis of 3T3-L1 cells. Figure S3. Adipogenesis time course of human iPSC-derived MSCs. Figure S4. FATS analysis of browning in subcutaneous fat-derived ASCs. Figure S5. High-throughput adipogenic screening assay of nuclear receptor ligands using human iPSC-derived MSCs was analyzed by FATS. Table S1. List of all the nuclear receptor ligands that were tested for high-throughput screening assay for adipogenesis in 3T3-L1 cells. Table S2. Results of high-throughput screening assay for adipogenesis of human iPS-derived MSCs in a single plate using nuclear receptor ligands library. Table S3. List of RT-qPCR primers-Oligos 5’ to 3’.(DOCX 5748 kb