Gata4 acts upstream of Hh signaling pathway.

(A-I’) Histology of Gata4 transgenic mouse embryo heart at E14.5. Two panel images of each embryonic heart were used to show the DORV phenotypes. The upper panels showed that the opening of AO connected to the right ventricle (C, DORV), or override on the ventricular septum (D and H, OA), or shared a common trunk with the PT which opens to the right ventricle (E, CAT or PTA) in the Gata4 haploinsufficency, while in control embryos the AO opened to the left ventricle (A, B, F, G and I). The lower panel showed the opening of PT connecting to the right ventricle (A’-I’). LV, left ventricle; RV, right ventricle; ao, aorta artery, pt, pulmonary trunk; CAT, common artery trunk. Magnification: 40X. (J-M’) H3S10 expression was detected in Gata4 transgenic mouse embryos by IHC at E9.5. Red rectangle area was amplified below. (J-M) Magnification: 100X. (J’-M’) Magnification: 200X. (J”-M”) Magnification: 400X. (N) Quantification of H3S10 labelled cells. Data is expressed as ratio to Gata4fl/+. Data is presented as Mean±SEM, ***pGata4fl/+ embryos. (O) Gene expression in the aSHF of Gata4fl/+, Gata4fl/+;Gli1-CreERT2/+ or Gata4fl/+;SmoM2fl/+;Gli1-CreERT2/+ embryos was measured by real-time PCR. Data is presented as Mean±SEM, n = 3, *P Gata4fl/+ embryos.