Read-through (RT) regions have reduced amino acid hydrophobicity compared with non-read-through (non-RT) regions.

Presented are the fractions of hydrophobic amino acids in the peptides conceptually translated from the last 16 codons of the coding region (CDS), region 1, and region 2 in 172 read-through genes and 6,399 non-read-through genes of the yeast (A) or 294 read-through genes and 11,389 non-read-through genes in the fruit fly (B). Error bars show standard errors determined by bootstrapping genes. One-tailed P-value is based on respectively bootstrapping genes of the two groups being compared.