Features of readthrough genes.

<p><b>A:</b> Scatterplot comparing readthrough and expression, including only those genes that passed the filters (see bioinformatics methods in supplementary information for description of filters). Points shown in red (709/2234) represent those genes showing significant readthrough in both experiments (FDR = 0.05). <b>B:</b> Polymerase occupancy around the 3' ends of 200 genes with the highest readthrough rank, and 1200 genes with the lowest rank. <b>C:</b> Polymerase occupancy around the 3' ends of genes, as in 7(B), measured in the <i>npl3</i>Δ background. <b>D:</b> Enrichment scores of 6-mer motifs in the 3’ region of low readthrough genes, relative to high readthrough genes. The 3’ region was defined as -80 to -20 nt from the polyadenylation site [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005735#pgen.1005735.ref037" target="_blank">37</a>]. Low and high readthrough genes were defined as the bottom and top quartiles of mRNAs in the dataset. UAUAUA and UAAAUA are the most enriched 6-mer motif in low-readthrough genes. <b>E:</b> Localization of UAUAUA motifs around the polyadenylation sites of low readthrough genes (red) and high readthrough genes (black), aligned by the polyA site.