CD4+ T cell functional profiles of vaccine specific responses in DAR-901 and BCG vaccinated individuals.

Side to side comparison of DAR-901 responses to DAR-901 lysate (n = 10) and BCG responses to BCG stimulant (n = 9). Pie charts represent relative proportions of respectivevaccine-specific CD4+ T cells co-producing combinations of A) IFNγ, TNFα and IL2, and B) combinations of IFNγ, IL2, IL17 and IL22 among the DAR-901 and BCG vaccinated subjects at the peak (7-days post-dose 3) and final time point (180-days post-dose 3). Frequencies of each gate were determined using Boolean gating. DAR-901 vaccine responses predominantly comprise of polyfunctional combinations of IFNγ+TNFα+IL2+ producing CD4+ T cells which were sustained until the final study point (Key: G = IFNγ, 2 = IL2, T = TNFα, 17 = IL17, 22 = IL22).