Expression of E- and N-cadherin in ESP-treated HuCCT1 and H69 cells.

HuCCT1 (A) or H69 (B) cells were treated with 800 ng/mL of ESPs and harvested after 0–48 hours for immunoblot analysis of E- and N-cadherin expression. (C) The 24-hour ESP-treated medium of HuCCT1 cells was replaced by the culture supernatants from each four different H69 siRNA transfectants as described in the materials and methods section. After a 48-hour-incubation, total soluble proteins were immunoblotted using polyclonal antibodies against E- and N-cadherin. Protein bands were quantified densitometrically and normalized to the density of the GAPDH band. The ratio of E- or N-cadherin to GAPDH in each group is presented as its fold-change relative to the non-treated 0–hour control (NC). *P P P t-test was used to analyze significance. Error bars = ± SEM.