EGF exposure induces EMT in Caski cells.

<p>Caski cells were cultured under routine conditions with or without EGF (50 ng/ml) for 1 to 5 days. A. The morphological changes caused by EGF were observed by microscopy. B. Downregulation of E-cadherin by EGF treatment was determined by western blot. Densitometric analysis of three independent triplicate Western blots. C. E-cadherin downregulation in response to EGF (50 ng/ml) treatment for 3 days was verified by IF. D. Upregulation of N-cad, MMP1 and annexin A2 in the EMT process as investigated by western blot. Densitometric analysis of three independent triplicate Western blots. Values are the average of triple determinations with the S.D. indicated by error bars. *P<0.05, ** P<0.01, compared to control.