Determination of THC in medical cannabis and biological samples : diploma thesis

Ciljevi istraživanja: Kvalitativno dokazati prisutnost THC-a i ostalih kanabinoida u medicinskim pripravcima i u biološkim uzorcima primjenom GC-MS metode, usporediti rezultate analize dobivene nakon pripreme uzoraka različitim ekstrakcijskim metodama (ekstrakcija čvrstom fazom, SPE, i ekstrakcija tekuće-tekuće, LLE) te utvrditi koja je metoda prikladnija za pripremu uzoraka pri analizi kanabinoida. Materijali i metode: Tri medicinska pripravka koji sadrže THC otopljena su i razrijeđena u kloroformu te analizirana GC-MS instrumentalnom metodom kako bi se utvrdio njihov sastav. Zatim su, koristeći te pripravke, pripravljena 24 biološka uzorka, od toga 12 uzoraka s mokraćom i 12 uzoraka sa serumom. Uzorci su podijeljeni u dvije istovjetne skupine i svaka je za analizu pripremljena različitom ekstrakcijskom metodom, ekstrakcijom čvrstom fazom (SPE) ili ekstrakcijom tekuće-tekuće (LLE). Uzorci su otopljeni u malom volumenu kloroforma i analizirani na GC-MS uređaju metodom koja omogućava istovremeno snimanje ukupnog ionskog kromatograma (TIC) u području od 40 – 600 m/z i snimanje samo odabranih iona (SIM).Objectives: Qualitative determination of THC and other cannabinoids in medical preparations and biological samples using GC-MS method, comparison of the results obtained after sample preparation with different extraction methods (Solid phase extraction, SPE, and Liquid-liquid extraction, LLE) and defining the more appropriate extraction method for sample preparation in cannabinoids analysis. Materials and methods: Three medical preparations which contain THC were dissolved and diluted in chloroform and analyzed with GCMS instrumental method in order to determine their composition. Then, that preparations were used to make 24 biological samples with different concentrations of cannabinoids, 12 samples of urine and 12 samples of serum. Samples were divided into two identical groups and each group was prepared for analysis using different extraction method, solid-phase extraction or liquid-liquid extraction. Samples were dissolved in a small volume of chloroform and analyzed with GC-MS method using mode that simultaneously records total ion chromatogram (TIC) in the area of 40-600 m/z and single ion monitoring (SIM) scanning mode