UHPLC-HRMS analysis of cannabinoids in biological matrix

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: RNDr. Martin Mžik, Ph.D. Candidate: Bc. Anna Ocelková Title of Thesis: UHPLC-HRMS analysis of canabinoids in biological matrix Cannabis is still considered to be the most widespread drug worldwide. The main molecule responsible for its effects is psychotropic cannabinoid Δ9 -tetrahydrocannabinol, which is converted into active metabolite 11-OH-Δ9 -THC and inactive metabolite 11-nor-9-carboxy-Δ9 -THC in the human body. The determination of these three molecules in human serum is one of the basic analytical assays that are performed in toxicological laboratories, as it reflects the state of current drug exposure. Another cannabinoid - cannabidiol (CBD) - is also gaining worldwide awareness, and its therapeutic effects have been the subject of many clinical studies. CBD has no psychoactive effect and its potential for the treatment of a wide range of diseases was first fully acknowledged with the launch of the first drug (Epidiolex®) on the US market in 2018 with CBD being the main active ingredient. The aim of this thesis is development and validation of the sensitive method for determination of Δ9 -THC,11-OH-Δ9 -THC, Δ9..