Avaliação de diferentes tipos de treinamento físico sobre o estresse oxidativo e parâmetros bioquímicos em diabetes mellitus tipo II e o efeito do exercício agudo sobre os biomarcadores salivares em atletas

CHAPTER II: Physical exercise induces biochemical changes in the body that modify analytes in blood and saliva among other body fluids. Many of these salivary protein analytes and their functions remain uncharacterized. This study analyzed the effect of an incremental effort test on the salivary protein profile to determine whether any specific protein is altered in response to such stress. We also measured thresholds of salivary alpha amylase, total salivary protein and blood lactate and searched for correlations among them. Twelve male cyclists underwent a progressive test in which blood and saliva samples were collected simultaneously at each stage. The salivary total protein profile revealed that physical exercise primarily affects the polypeptide corresponding to salivary alpha-amylase, the concentration of which increased markedly during the test. We observed thresholds of salivary alpha-amylase (sAAT), total salivary protein (PAT) and blood lactate (BLT) in 58%, 83% and 100% of our sample, respectively. Pearson s correlation indicates,during the test, a strong and significant association between sAAT and BLT (r= 0.84 p<0.05), sAAT and PAT (r= 0.83 p<0.05) and BLT and PAT (r= 0.90 p