A field trial was conducted for 3 yr (2005 through 2007) near Scottsbluff, NE, to examine weed control, crop safety, forage production, and economics of glyphosate-tolerant and conventional alfalfa establishment systems. Glyphosate applied to alfalfa at the unifoliate growth stage provided 67% weed control and was similar to imazamox applied at the two-trifoliate leaf stage. Delaying glyphosate application until alfalfa had reached the two-trifoliate growth stage improved weed control to 83%, and weed control was similar to imazamox plus 2,4-DB and imazethapyr plus 2,4-DB. Imazamox and imazethapyr caused minor crop injury, and the addition of bromoxynil or 2,4-DB to both herbicides further decreased crop safety. Weeds were most competitive ...