Citation: Paper, J. M., Mukherjee, T., & Schrick, K. (2018). Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants. Plant Methods, 14(1). https://doi.org/10.1186/s13007-018-0299-2Background: Phospholipids are important structural and signaling molecules in plant membranes. Some fluorescent dyes can stain general lipids of membranes, but labeling and visualization of specific lipid classes have yet to be developed for most components of the membrane. New techniques for visualizing membrane lipids are needed to further delineate their dynamic structural and signaling roles in plant cells. In this study we examined whether propargylcholine, a bioortholog of choline, can be used to label the major membrane li...
© 2021 Cheka Lumbini KehelpannalaLipids are important constituents of plants, performing many fundam...
Diacylglycerol (DAG) is an intermediate in metabolism of both triacylglycerols and membrane lipids. ...
The biogenesis of cellular membranes involves an important traffic of lipids from their site of synt...
Citation: Paper, J. M., Mukherjee, T., & Schrick, K. (2018). Bioorthogonal click chemistry for fluor...
Eukaryotic cells are surrounded by membranes consisting of various lipids, including sterols, sphing...
Chromatography (GLC). J. Vis. Exp. (49), e2518, doi:10.3791/2518 (2011). Biological membranes separa...
Additional file 5: Table S3. Mole percent of choline phospholipids in propargylcholine-treated and u...
Additional file 4: Table S2. Levels (nmol/mg dry weight) of choline phospholipids in propargylcholin...
Additional file 7: Table S5. Mole percent of non-choline lipids in propargylcholine-treated and untr...
KEY MESSAGE: The present work demonstrates the use and advantages of novel, live cell permeable, li...
Additional file 6: Table S4. Levels (nmol/mg dry weight) of non-choline lipids in propargylcholine-t...
Living cells delimit themselves from their surroundings by a biological membrane. This biological me...
Improvements in mass spectrometry (MS)-based strategies for characterizing the plant lipidome throug...
Additional file 2: Figure S2. Comparison of Alexa Fluor 594 azide signals from untreated controls wi...
Background: Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types thro...
© 2021 Cheka Lumbini KehelpannalaLipids are important constituents of plants, performing many fundam...
Diacylglycerol (DAG) is an intermediate in metabolism of both triacylglycerols and membrane lipids. ...
The biogenesis of cellular membranes involves an important traffic of lipids from their site of synt...
Citation: Paper, J. M., Mukherjee, T., & Schrick, K. (2018). Bioorthogonal click chemistry for fluor...
Eukaryotic cells are surrounded by membranes consisting of various lipids, including sterols, sphing...
Chromatography (GLC). J. Vis. Exp. (49), e2518, doi:10.3791/2518 (2011). Biological membranes separa...
Additional file 5: Table S3. Mole percent of choline phospholipids in propargylcholine-treated and u...
Additional file 4: Table S2. Levels (nmol/mg dry weight) of choline phospholipids in propargylcholin...
Additional file 7: Table S5. Mole percent of non-choline lipids in propargylcholine-treated and untr...
KEY MESSAGE: The present work demonstrates the use and advantages of novel, live cell permeable, li...
Additional file 6: Table S4. Levels (nmol/mg dry weight) of non-choline lipids in propargylcholine-t...
Living cells delimit themselves from their surroundings by a biological membrane. This biological me...
Improvements in mass spectrometry (MS)-based strategies for characterizing the plant lipidome throug...
Additional file 2: Figure S2. Comparison of Alexa Fluor 594 azide signals from untreated controls wi...
Background: Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types thro...
© 2021 Cheka Lumbini KehelpannalaLipids are important constituents of plants, performing many fundam...
Diacylglycerol (DAG) is an intermediate in metabolism of both triacylglycerols and membrane lipids. ...
The biogenesis of cellular membranes involves an important traffic of lipids from their site of synt...