Detection of autophagic vacuoles by transmission electron microscopy in cells transfected with full-length HBV genomes.
- María Mercedes Elizalde (5184335)
- Paula Soledad Pérez (5184341)
- Ina Sevic (715000)
- Daniel Grasso (132388)
- Alejandro Ropolo (5184344)
- Luciana Barbini (132430)
- Rodolfo Héctor Campos (3150381)
- María Inés Vaccaro (5184338)
- Diego Martín Flichman (3150393)
Publication date
May 2018
DOIAbstract
<p>(A) Representative transmission electron micrographs of Huh-7 cells transfected with pUC19 empty vector (control), F1b wt, F1b BCPdm, F1b preCore, F4 wt, F4 BCPdm and F4 preCore variants. Arrows indicate representative autophagic vacuoles. Inset: enlargement of autophagic vacuoles in the electron micrograph. (B) Quantification of autophagic vacuoles (autophagosomes and autolysosomes). Shown values represent the mean ± standard deviation of three independent experiments. Statistically significant changes compared to control cells are indicated with asterisks above bars and statistically significant changes between the viral variants are indicated with asterisks above brackets. *** <i>p</i> < 0.0001.</p
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