Stress fiber formation in response to SmoA1 and Gα₁₃QL.

<p>C3H10T1/2 cells grown on coverslips were serum-starved for 12 h then incubated with recombinant adenoviruses encoding LacZ, SmoA1, or Gα₁₃QL (MOI = 10) in the presence of DEAE-Dextran (10 μg/ml). They were serum-starved an additional 18 h and fixed with paraformaldehyde. F-actin was stained with FITC-Phalloidin, and nuclei were counterstained with DAPI. ‘Merge’ is pseudocolored. Shown is one experiment representative of three total. Few or no stress fibers were identified in the absence of transduction (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0197442#pone.0197442.s002" target="_blank">S2 Fig</a>).