Dissolution of actin stress fibers in cells expressing RIT1 and NS-associated RIT1 mutants.

<p>(A) COS7 cells were plated on collagen-coated glass slides, transiently transfected with the indicated constructs and kept under serum starvation overnight. HA-tagged RIT1 was stained by rabbit anti-HA antibody followed by anti-rabbit Alexa Fluor488-conjugated antibody. Polymerized F-actin was visualized using Texas Red-X Phalloidin, and nuclear DNA was labeled by DAPI. Cells were imaged by epifluorescence microscopy. White boxes indicate magnified parts of specimen shown on the very right-hand side. Scale bars, 10 μm. (B) Cells were divided into the two indicated categories: (I) normal and (II) reduced or absent actin stress fibers (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007370#pgen.1007370.s009" target="_blank">S8B Fig</a> shows exemplary images). A minimum of 50 cells per dataset were analyzed.