Mitochondrial fragmentation is involved inpancreatic beta cell death in hypoxia conditions.

<p><b>A.</b>Detection of DRP1 (S616) and mitochondrial-fractional DRP1by western blot analysis in pancreatic beta INS-1E cellswith treatment as indicated. <b>B.</b>Detection of apoptosis by flow cytometry in pancreatic beta INS-1E cellswith treatment as indicated. <b>C.</b>Western blot analysis for levels of cytochrome c inpancreatic beta INS-1E cellswith treatment as indicated. β-actin and VDAC were used as loading controls for cytoplasm and mitochondria, respectively. Cyto, cytoplasm; Mito, mitochondrial. <b>D.</b>Western blot analysis for levels ofcleaved Caspase-9 and Caspase-3 inpancreatic beta INS-1E cellswith treatment as indicated. <b>E.</b>TUNEL staining in pancreatic beta INS-1E cells from type 2 diabetes animal models. Blue: Hoechst 33342; Green: TUNEL positive nucleus. All experiments were repeated three times.*<i>P</i>< 0.05.