Expression of S1PR2 in the lung of <i>S1pr2</i>^LacZ/+ mice.

<p>(A) X-gal stained brain, heart, lung, liver, spleen and kidney from 8-week old WT and <i>S1pr2</i>^LacZ/+ mice. (B) X-gal stained lung sections of <i>S1pr2</i>^LacZ/+ mice on day 33 after starting administration of saline or bleomycin (Bleo). Magnification: x400. Scale bar: 100 μm. (C) X-gal stained and anti–Mac3 immunostained lung sections of <i>S1pr2</i>^LacZ/+ mice on day 33. Magnification: x1000. Scale bar: 100 μm. (D) X-gal stained alveolar macrophages in the BALF smear from bleomycin–administered <i>S1pr2</i>^LacZ/+ mice. Magnification, x1000. Scale bar, 100 μm. In (B) and (D), the tissue sections were counterstained by nuclear fast red. In (C) and (D), the arrowheads denote X-gal-stained macrophages. The presented data represent three independent experiments.