Additional file 1: of Wheat CBL-interacting protein kinase 23 positively regulates drought stress and ABA responses

Figure S1. Phylogenetic tree of CIPK proteins from rice, soybean, rapeseed, Aegilops tauschii, Triticum urartu, maize, sorghum and Arabidopsis . The phylogenetic tree was constructed based on the sequence alignments. The phylogenetic tree of CIPK proteins was constructed with MEGA5.1 program with the neighbor-joining method. The numbers beside the branches represent bootstrap values based on 1000 replications. Figure S2. The expression level of drought-responsive genes was altered in TaCIPK23- overexpressing plants under drought conditions. Two-week-old TaCIPK23-overexpressing and wild-type seedlings with 6% PEG6000 treatment were used for RNA isolation. Transcript level was quantified by qRT-PCR assays. Expression of Actin was analyzed as a control. Each data point is the mean (ÂąSE) of three experiments (10 seedlings per experiment). Table S1. Primers used for qRT-PCR assays. (PDF 496Â kb