Tsetse fly midgut infection rate with the Δ<i>akct</i>, Δ<i>ivdh</i>-C3 and Δ<i>ivdh</i>-C8 cell lines.

<p>In this experiment, 50 to 100 <i>Glossina morsitans morsitans</i> teneral males were artificially fed with either the parental (PCL), Δ<i>akct</i>, Δ<i>ivdh</i>-C3 or Δ<i>ivdh</i>-C8 PCF cell lines in culture medium as previously described (n = 850) [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007116#ppat.1007116.ref041" target="_blank">41</a>]. Two weeks after the infective meal, all living flies were dissected to assess the presence of parasites in their entire midgut by microscope examination. Midgut infection rates (in % ±SD) are presented for each strain as the mean of three (mutant cell line) or six (PCL) independent biological replicates (n = 243 for PCL, n = 169 for Δ<i>akct</i>, n = 78 for Δ<i>ivdh</i>-C3, n = 65 for Δ<i>ivdh</i>-C8).