Delayed removal of COSA-1 foci in <i>mus-81 lem-3</i> double mutants.

<p>(A) Crossover designation is normal in <i>mus-81 lem-3</i> and <i>lem-3; slx-4</i> double mutants. DAPI staining of representative pachytene nuclei containing GFP::COSA-1 foci. Scale bars: 2 μm. (B) Quantification of nuclei with indicated number of COSA-1 foci. (C) Projections of representative nuclei from diakinesis oocytes of wild type, <i>lem-3</i>, <i>mus-81</i>, <i>slx-4</i>, <i>mus-81 lem-3</i> and <i>lem-3; slx-4</i> mutants stained with α-HTP-3, a component of the <i>C</i>. <i>elegans</i> axial element (red) and DAPI (blue). The persistent GFP::COSA-1 foci localized between two homologous pairs are highlighted by white arrowheads. (D) Scheme depicting normal bivalent from wild type and dissociated bivalent with COSA-1 focus at junction from <i>slx-4</i> mutant at the diakinesis stage. (E) Quantification of the diakinesisi nuclei with indicated number of COSA-1 foci. The sample size (n) indicates the number of germline examined for each genotype.