Comparison of diakinesis chromosome bivalents in wild type and <i>mus-81; xpf-1</i> animals.

<p>(A) Representative DAPI-stained -1 oocytes illustrating differences in the morphology of wild type and <i>mus-81; xpf-1</i> bivalents. (B) Representative images illustrating fine DNA bridges between the DAPI-stained bodies in <i>mus-81; xpf-1</i> animals. (C) AIR-2 antibody staining in −1 oocytes in wild type and <i>mus-81; xpf-1</i> animals. Note how AIR-2 staining flanks the DNA bridge regions. (D) Representative images of antibody staining of the synaptonemal complex axial element protein HTP-3 in −1 oocytes. Note that the bivalents in <i>mus-81; xpf-1</i> do not exhibit the wild type cruciform pattern for HTP-3 staining suggesting that the synaptonemal complex and bivalent architecture is disorganized in double mutant. Bars 2 µm.