m⁶A depletion induces dramatic gene expression changes in the cerebellum.

<p>(A) The most enriched sequence motif of m⁶A peaks in mRNAs from P7 mouse cerebellums. (B) Distribution of m⁶A peaks along the 5′UTR, CDS, and 3′UTR regions of total cerebellar mRNAs from P7 Ctrl mice after normalized with length. (C) Venn diagram representing the relationships between cerebellar expressed genes and m⁶A modification. Green circle represents all expressed genes in P7 Ctrl mouse cerebellums, brown circle represents genes with m⁶A modifications in P7 Ctrl mouse cerebellums, red circle represents genes with up-regulated expression in P7 <i>Mettl3</i> cKO mouse cerebellums as compared to the Ctrl, and blue circle represents genes with down-regulated expression in P7 <i>Mettl3</i> cKO mouse cerebellums as compared to the Ctrl. Numbers represent the counts of genes in each group. (D) Significantly enriched (<i>p</i>-value ≤ 0.01, Benjamini–Hochberg multiple testing correction) GO terms of genes with up-regulated expression in P7 cKO cerebellums and with m⁶A peaks. CDS, coding sequence; cKO, <i>Mettl3</i> conditional knockout; GO, gene ontology; JAK-STAT, Janus kinase (JAK), Signal Transducer and Activator of Transcription protein (STAT); m⁶A, <i>N</i>⁶-methyladenosine.