<p>Purified VLPs were fixed for 24h at room temperature with formaldehyde, contrasted with phosphotungstic acid and analyzed by transmission electron microscopy.</p
<p>VP1 were produced using recombinant baculoviruses. The preparations were applied to carbon-coated...
<p>293T cells were cotransfected with SARS-CoV N and wt or indicated M mutant plasmid. At 48 h post-...
<p>Purified Mo-MLV VLPs were pelleted and prepared for TEM. (A–D) Representative electron micrograph...
<p>VLP were gradient-purified, negatively stained and visualized at 30,000-fold magnification using ...
<p>(A) Transmission electron micrographs of HPV16 VLPs in the initial (left) and final states (right...
<p>A – HBc1-183, B – HBc1-161, C – HBc1-152, and D – HBc1-141. Scale bar, 50 nm.</p
<p>Partially purified VLPs were negatively stained with 0.5% aqueous uranyl acetate and visualized b...
<p>All the HPV16 VLP preparations were finally dialyzed against 0.325 M NaCl in phosphate buffer pH ...
<p>(A) Quantities of L1 protein in cell lysates were compared by Western blotting. (2 μg of cell lys...
Formaldehyde (FA) fixation of infectious samples is a well-established protocol in diagnostic electr...
<p>To analyze the structural integrity of the two VLP types, the HPV16 VLPs were fractionated on Opt...
<p>Extracts from infected cells at the optimal production times with each baculovirus were processed...
<p>Thin section electron micrographs were immunogold stained for Env using the PGT145 and PGT151 Ab ...
<p><b>a</b> After coupling of the mSA-ID1-ID2a antigen to the HPV16 Avi-L1 VLPs, excess antigen was ...
<p>(A) Negative stain electron microscopy of FCV VLP particles from insect cell culture supernatants...
<p>VP1 were produced using recombinant baculoviruses. The preparations were applied to carbon-coated...
<p>293T cells were cotransfected with SARS-CoV N and wt or indicated M mutant plasmid. At 48 h post-...
<p>Purified Mo-MLV VLPs were pelleted and prepared for TEM. (A–D) Representative electron micrograph...
<p>VLP were gradient-purified, negatively stained and visualized at 30,000-fold magnification using ...
<p>(A) Transmission electron micrographs of HPV16 VLPs in the initial (left) and final states (right...
<p>A – HBc1-183, B – HBc1-161, C – HBc1-152, and D – HBc1-141. Scale bar, 50 nm.</p
<p>Partially purified VLPs were negatively stained with 0.5% aqueous uranyl acetate and visualized b...
<p>All the HPV16 VLP preparations were finally dialyzed against 0.325 M NaCl in phosphate buffer pH ...
<p>(A) Quantities of L1 protein in cell lysates were compared by Western blotting. (2 μg of cell lys...
Formaldehyde (FA) fixation of infectious samples is a well-established protocol in diagnostic electr...
<p>To analyze the structural integrity of the two VLP types, the HPV16 VLPs were fractionated on Opt...
<p>Extracts from infected cells at the optimal production times with each baculovirus were processed...
<p>Thin section electron micrographs were immunogold stained for Env using the PGT145 and PGT151 Ab ...
<p><b>a</b> After coupling of the mSA-ID1-ID2a antigen to the HPV16 Avi-L1 VLPs, excess antigen was ...
<p>(A) Negative stain electron microscopy of FCV VLP particles from insect cell culture supernatants...
<p>VP1 were produced using recombinant baculoviruses. The preparations were applied to carbon-coated...
<p>293T cells were cotransfected with SARS-CoV N and wt or indicated M mutant plasmid. At 48 h post-...
<p>Purified Mo-MLV VLPs were pelleted and prepared for TEM. (A–D) Representative electron micrograph...