SARS-CoV VLP analysis.

<p>293T cells were cotransfected with SARS-CoV N and wt or indicated M mutant plasmid. At 48 h post-transfection, cells and supernatants were collected. Cells (panels A to I) were fixed and prepared for electron microscopy analysis as described in Material and Methods. Supernatants (panels J to L) were filtered and pelleted through 20% sucrose cushions, resuspended in PBS buffer and stained. Cell and supernatant samples were observed with a transmission electron microscope. The high-power view in the inset shows VLPs near cell nuclei (arrowheads). Bars, 100 nm.