C₂G₄ repeat-length dependence on the formation of iCD-DNA.

<p>CD spectra of d(C₂G₄)_n (where n = 2–7), in 150 mM lithium citrate, pH 5.2, at DNA concentrations adjusted to ensure unvarying DNA mass from solution to solution. Each DNA was incubated at 700 μM concentration in 150 mM lithium citrate, pH 5.2, at 37°C for 14 hrs, following which it was diluted to ensure constant DNA mass into the same buffer as follows—“7”: 2.85 μM d(C₂G₄)₇; “6”: 3.33 μM d(C₂G₄)₆; “5”: 4.0 μM d(C₂G₄)₅; “4”: 5.0 μM d(C₂G₄)₄; “3”: 6.66 μM d(C₂G₄)₃; and “2”: 10.0 μM d(C₂G₄)₂.