Formation of iCD-DNA.

<p>(a) Circular dichroism spectra of 20 μM d(C₂G₄)₇ in TE Buffer plus 150 mM LiCl, pH 7.4. 700 μM DNA in this buffer, at 37°C, was incubated for 14 hrs and for 5 days. CD spectra were taken shortly following dilution to 20 μM DNA, in the same buffer, and measured at 22°C. (b) Circular dichroism spectra of 20 μM d(C₂G₄)₇ in 150 mM lithium citrate buffer at different pH (5, 5.2, 5.4, 5.6, 6) as well as in TE buffer plus 150 mM LiCl (at pH 7.0 and 7.4). 700 μM DNA, in the above buffers, was incubated for 14 hrs at 37°C. CD spectra were taken shortly after dilution to 20 μM DNA, in the appropriate buffer, and measured at 22°C. (c) θ₂₈₀ from Fig 1B plotted as a function of pH.