Phosphoproteomic analysis of a MEKK1 null mutant.

<p><b>A.</b> Experimental approach. <i>T</i>. <i>brucei</i> EATRO 1125 AnTat1.1 90:13 and <i>MEKK1</i> null mutants were grown in culture and harvested after identical growth profiles and at low cell density to maintain them as the slender developmental form. Samples were isolated, processed, subjected to isobaric mass tagging and then analysed by LC MS/MS. <b>B.</b> Reproducibility of the identified protein and phosphoprotein profiles derived from the replicates of the parental (‘AnTat1.1’) and <i>MEKK1</i> null mutant cells. <b>C.</b> Volcano plot analysis of phosphopeptide changes between the parental and <i>MEKK1</i> null mutant. Analyses focused on changes that were statistically significant (adjusted P value <0.05; shown with red dashed lines on the–log10 scale) and at least 1.5-fold different (shown as blue dashed lines on the Log2 scale) between the parental and <i>MEKK1</i> null mutant.