Differential gene expression analysis in wild type and <i>TbCMT1</i> null mutant cells.

<p>The figure shows the log10 transformation of the normalized mean for the transcript reads detected in the WT and <i>TbCMT1</i> null mutant samples computed by DESeq2 (Base Mean, x axis) versus the log2 transformation of their mean fold change (y axis). A positive fold change indicates transcript upregulation and a negative fold change indicates downregulation in the <i>TbCMT1</i> null samples relative to WT. Highlighted with arrows are the top 5 up-regulated VSG-related transcripts (VSGs) and top 3 down-regulated cysteine peptidases transcripts (Cysteine Peptidases). The plot also highlights the position of the top downregulated VSG transcript (VSG), the position of the downregulated <i>TbCMT1</i> transcript (TbCMT1) and the position of the unchanged MITat1.2 VSG transcript. (B) The plot shows the quantification of the Cathepsin-L protein determined by quantitative Western blot in wild type (WT) and three <i>TbCMT1</i> null mutants clones (null-1, null-2 and null-3). The values of the Cathepsin-L protein are normalized to the HSP-70 protein and divided by the value for the WT sample.