Generation of conditional <i>Ssrp1</i> KO mice.

<p>A) Schematic representation of the wild-type <i>Ssrp1</i> allele (<i>Ssrp1</i>⁺) and the targeting vector with 5’ and 3’ homology arms (pink dashed lines). The black dashed lines indicate intron regions in <i>Ssrp1</i>⁺ where LoxP sites, FRT sites, and the synthetic cassette are inserted. B) Schematic representation of the mutant allele (<i>Ssrp1</i> ^{<i>Neo LacZ fl</i>}) after homologous recombination, representation of mutant allele without the synthetic cassette (<i>Ssrp1</i>^<i>fl</i>) after Flp-FRT recombination and representation of <i>Ssrp1</i>^<i>Δ</i> allele with deletion of the critical exons after CreER^T2-LoxP recombination. C) Southern blot hybridization of DNA from ESC cells to determine clones with correct (red) and incorrect (black) recombination of 3’ and 5’ arm. Wild-type DNA used as control for 5’ probe is C. D) PCR of genomic DNA from F1 progeny of wild-type (<i>Ssrp1</i>^<i>+/+</i>) crossed with chimera (<i>Ssrp1</i> ^{<i>Neo LacZ fl /</i>+}). Two bands are present in heterozygous pups (<i>Ssrp1</i> ^{<i>Neo LacZ fl</i> /+}) (red). Numbers indicate individual pup IDs in one litter. E) β-Galactosidase assay to determine LacZ activity (Blue-Green stain for positive activity) on SSRP1 positive (colon, pancreas and testis) and negative (lung) tissues isolated from <i>Ssrp1</i>^+/+ and <i>Ssrp1</i> ^{<i>Neo LacZ fl</i> /+} mice.