The predicted kinetics.

<p>The time dependence of the apparent phosphoryl transfer rate constants for the two different dual-regulation mechanisms (<i>H</i>₆ and <i>H</i>₇) are represented by the two different colors (blue for regulating and (<i>H</i>₇) and orange for regulating and (<i>H</i>₆)). The system studied here contains the least active receptors (EEEE) in membrane vesicles with [Asp] = 10 <i>μ</i>M, [ATP] = 10⁴ <i>μ</i>M, and [P1] = 400 <i>μ</i>M, which reproduces the conditions of the rightmost point of the lower curve in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1006305#pcbi.1006305.g004" target="_blank">Fig 4(d)</a>. The solid and the dashed lines correspond to the instantaneous (<i>k</i>) and the average () phosphorylation rate constants respectively. The black circle is the experimental data point from [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1006305#pcbi.1006305.ref017" target="_blank">17</a>].