Microglial density and TNFα production in <i>ATXN1[82Q]; IKKβ</i>^<i>F/F</i><i>;LysM Cre</i> mice.

<p><b>A.</b> Cerebella of 3-month–old mice were used for IHC with Iba1-antibody. Representative image of microglia specific Iba-1 staining. <b>Insets of</b> higher magnification images illustrating microglial morphology. <b>B.</b> Quantification of microglial density in the molecular layer (N ≥ 4 per each genotype), one-way ANOVA followed by Tukey’s HSD post-hoc test. * P < 0.05 <b>C.</b> RNA was extracted from the cerebella of 3-month–old mice and RT-qPCR was used to determine expression of TNFα (with reference to control treated wild type and normalized to 18S RNA). Each dot represents one mouse, and values indicate mean ± SEM, one-way ANOVA followed by Bonfferoni post-hoc test. * P < 0.05, ** P < 0.01, *** P < 0.0001. Open bars are WT controls, light blue bars are <i>ATXN1[82Q]</i> mice, and hashed bars indicate the presence of <i>IKKβ</i>^<i>F/F</i><i>;LysM Cre</i>.