Spinal cord microglia in control and hSOD1^G93A mice at a pre-symptomatic stage.

<p>The number of microglia from hSOD1^G93A animals and their control littermates was assessed by flow cytometry using the microglial marker Iba1. (A–C) - Representative flow cytometry analysis dot plot microglia profiles. A - Negative control (without Iba1 staining). B - Control and C - hSOD1^G93A spinal microglia at P30. In both B - controls and C - hSOD1^G93A surrounded areas, designed as “P3", correspond to the labeled cells. D - Quantification of Iba1⁺-microglia in control and hSOD1^G93A animals at pre-symptomatic (P30) age. (E–F) - Photographs of Iba1 immunohistochemical labeling from E - a control or F - hSOD1^G93A lumbar spinal cord section. The X-axis represents the intensity of fluorescence and the Y-axis the size of the cells. Statistics: t-test; *** p<0.001. Scale bars 50 µm.