Additional file 2: of Vitamin C increases 5-hydroxymethylcytosine level and inhibits the growth of bladder cancer

Figure S2. (A) The relative transcription levels measured by RT-qPCR of TET1/2/3 in T24 cells treated with or without vitamin C. Statistical significance was determined by the Mann-Whitney U test. (B) Western blot of apoptosis markers for T24 cells at varying vitamin C concentrations. (C) Cell viability measured with MTS of T24 and 5637 cells at varying vitamin C concentrations with or without catalase at 100Â Îźg/ml. Statistical significance was determined by the Mann-Whitney U test. (D) Venn diagrams showing the overlap between decreased 5hmC peak-associated genes in bladder cancer and differentially expressed genes (left); KEGG pathway analysis results for overlapping genes (right). (E) Venn diagrams showing the overlap between decreased 5hmC peak-associated genes in bladder cancer; increased 5hmC peak-associated genes after vitamin C treatment, and differentially expressed genes (left); KEGG pathway analysis results for overlapping genes (right). (TIF 980Â kb