Proteins interacting with PABP1 and PABP2.

<p>A high confidence list of PABP interacting proteins was obtained by excluding proteins with an enrichment score less than two-fold in all four replicates; moreover, maximal one infinite ratio was tolerated. <b>(A)</b> Proteins co-precipitating with PABP1 (blue) or PABP2 (red) are shown sorted according to their average enrichment rates. Note the differences in scale on the y-axis between the two PABPs. <b>(B)</b> Differences between the interactomes of PABP paralogs; all 27 proteins that are enriched in either or both PABP pull-downs are ranked according to enrichment ratio PABP1/PABP2. Bait proteins are shown in yellow. Proteins unique to either PABP, defined by having an enrichment ratio <2 in the other PABP, are shown as large circles. Localisation to starvation stress granules is indicated for each protein (see <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006679#pntd.0006679.s001" target="_blank">S1G Table</a> for references). Stress granule localisation information based on experimental data are shown in dark colours, other evidence (from TrypTag data [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006679#pntd.0006679.ref059" target="_blank">59</a>]) is in lighter colours. <b>(C)</b> Reverse immunoprecipitations: eIF4E4 or G1-IP2 was expressed as an eYFP fusion protein together with either PABP1-4Ty1 or PABP2-4Ty1, all from their endogenous locus. eIF4E4 and G1-IP2 were precipitated from cryo-milled powders with recombinant anti-GFP Llama antibodies. Co-precipitated PABP proteins were detected with anti-Ty1 (BB2) monoclonal antibody. Beads without antibody were employed to calculate the enrichment factor. Data from two replicates (exp1 and exp2) are shown, and one representative blot.