Molecular phylogenetic analysis using Maximum Likelihood.

<p>The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Joining and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 72 nucleotide sequences. All positions containing gaps and missing data were eliminated. Evolutionary analyses were conducted in MEGA 6 software [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006675#pntd.0006675.ref022" target="_blank">22</a>]. Isolates of this study are marked with a filled circle. ITS Sequences of <i>Fonsecaea brasiliensis</i>, <i>Fonsecaea erecta</i>, and <i>Fonsecaea multimorphosa</i> were used as outgroups.