Molecular Phylogenetic analysis by Maximum Likelihood method based on the Kimura two-parameter model of the WNV sequences of amplified fragments and homologous fragment obtained in GenBank library.

<p>The tree with the highest log likelihood (−720.3421) is shown. Initial tree for the heuristic search were obtained automatically by Neighbor-joining method with Maximum Composite Likelihood distance matrix. The analysis involved 22 nucleotide sequences, there were a total of 409 positions in the final dataset. Evolutionary analyses were conducted in MEGA5 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038058#pone.0038058-Tang1" target="_blank">[65]</a>, the tree was rooted in RO97 50. Percentages of bootstrap test (1000 replicates) are shown next to the branches.