Additional file 7: of Additive contributions of melanopsin and both cone types provide broadband sensitivity to mouse pupil control

Figure S7. Additional validation of cone-isolating stimuli. (a) Mean ± SEM responses of colour opponent and non-opponent MR and non-MR units in Opn1mwR and Opn1mwR;Opn4−/−mice that responded at ND1 (conventions as in Fig. 3b). Data were analysed by one-way RM ANOVA with Dunnett’s post-test (clockwise from top left n = 19, 18, 7, 17, 36 and 15). (c) Top: Mean ± SEM responses of Cnga3−/−, Opn1mwR and Opn1mwR;Opn4−/− neurons to 75% contrast cone-isolating stimuli; (analysis includes all light-responsive cells tested in all genotypes; n = 24, n = 230 and n = 41 respectively). Bottom: cumulative frequency distribution for maximal response evoked by cone-isolating stimuli in the same populations of cells. Data were analysed by Kruskal-Wallis test with Dunn’s test for multiple comparisons. *,**,*** indicate P < 0.05, P < 0.01 and P < 0.001 respectively; ns indicates P > 0.05. (c) Changes in cone-opsin contrast for cone-silent stimuli as a result of varying both λmax and contribution of penumbral cones (conventions as in Fig. 3f, i). (JPG 135 kb