Additional file 8: of Additive contributions of melanopsin and both cone types provide broadband sensitivity to mouse pupil control

Figure S8. Opponent and non-opponent responses of pretectal neurons from Opn1mwR;Opn4−/− mice under the stimulus paradigm used for pupillography. (a, b) Mean ± SEM baseline subtracted responses of S-ON/L-OFF (a; n = 6) and non-opponent (b; n = 18) Opn1mwR;Opn4−/− units (from 4 mice) to 75% contrast cone isolating and all opsin stimuli delivered as for pupillography (Fig. 5). (c, d) Left: Mean ± SEM change in firing between ‘dim’ and ‘bright’ stimulus phases for all stimuli (averaged across full 5 s phase and both stimulus polarities) for S-ON/L-OFF (c) and non-opponent units (d) as above. Right: Mean ± SEM change in firing between ‘dim’ and ‘bright’ stimulus phases for 75% contrast stimuli targeting L + S cone opsins or all photoreceptors as a function of time since contrast step (averaged across both stimulus polarities as above). Data analysed by two-way RM ANOVA with Sidak’s post-tests. Non-opponent units lacked any stimulus-related differences, but a nominally significant increase in S-ON/L-OFF neuronal responses at 3 s but not earlier or later timepoints was observed (P = 0.03). (JPG 294 kb