Gallibody binding to natural MAs in ELISA.

<p><b>Coating of MA antigens was done at 250</b> μ<b>g/ml in hexane.</b> Hexane alone was used as a negative antigen control for background correction of the data. A) Anti-MA 12, B) Anti-MA 16, C) Anti-MA 18. Error bars = Standard deviation, n = 8 (biological repeats), CH1-4 = full length constant region, CH2-4 = truncated constant region. This result is representative of two biological repeats with four technical repeats for each concentration of gallibody. Insert A1) represents gallibody 12_CH2-4 titration at lower concentrations. No non-specific binding of gallibodies was observed when the antigen negative control was used. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0200298#pone.0200298.s004" target="_blank">S2 Dataset</a>.