Characterization of the binding specificities of MA-specific phage-displayed Abs with ELISA using single stereo-isomers of stereo-controlled synthetic MAs, their 1:1 mixtures with α-MA and natural MA mixture.

<p>In this monoclonal phage ELISA assay, the coating of MA antigens was done by adding 50 μl per well of MA (62.5 μg/ml) dissolved in hexane. Hexane alone was used as a negative control. A) Anti-MA 12, B) Anti-MA 16, C) Anti-MA 18. Error bars = Standard error of mean, n = 8 (biological repeats) t = <i>trans</i>, c = <i>cis</i>. The experiments on all synthetic MAs were performed with the three mAbs in parallel under the same conditions. This result is a representative of more than three biological repeats with six technical repeats for each mAb. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0200298#pone.0200298.s003" target="_blank">S1 Dataset</a>.