The Significance of Discordant Serology in Chagas Disease: Enhanced T-Cell Immunity to Trypanosoma cruzi in Serodiscordant Subjects
- Castro Eiro, Melisa Daiana
- Alvarez, María G.
- Cooley, Gretchen
- Viotti, Rodolfo Jorge
- Bertocchi, Graciela L.
- Lococo, Bruno
- Albareda, María Cecilia
- de Rissio, Ana María
- Natale, Maria Ailen
- Parodi Ramoneda, Cecilia María
- Tarleton, Rick L.
- Laucella, Susana Adriana
DOIAbstract
Background: Subjects are considered infected with Trypanosoma cruzi when tested positive by at least two out of three serological tests, whereas a positive result in only one of up to three tests is termed "serodiscordant" (SD). Assessment of parasite-specific T-cell responses may help discriminate the uninfected from infected individuals among SD subjects. Methods: Peripheral blood mononuclear cells from SD and seropositive (SP) subjects, who were born in areas endemic for T. cruzi infection but living in Buenos Aires city, Argentina, at the time of the study, and seronegative unexposed subjects were included for analysis. The function and phenotype of T cells were assessed by interferon-γ (IFN-γ) and interleukin (IL)-2 enzyme-linked immun...
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