Spray dried microspheres based on chitosan: A promising new carrier for intranasal administration of polymeric antigen BLSOmp31 for prevention of ovine brucellosis
- Díaz, Alejandra Graciela
- Quinteros, Daniela Alejandra
- Llabot, Juan Manuel
- Palma, Santiago Daniel
- Allemandi, Daniel Alberto
- Ghersi, Giselle
- Zylberman, Vanesa
- Goldbaum, Fernando Alberto
- Estein, Silvia Marcela
DOIAbstract
Previous studies have demonstrated that parenteral immunization with polymeric antigen BLSOmp31 induced a strong immune response and conferred protection against Brucella ovis in rams. This work describes the development of a novel formulation strategy for the delivery of BLSOmp31 in the nasal mucosa. Chitosan microparticles were prepared by spray-drying technology processes and recombinant chimera BLSOmp31 was loaded by passive adsorption onto chitosan microspheres, which were characterized by means of the evaluation of size, zeta potential, morphology, and loading and release rate of BLSOmp31. The mucoadhesive properties of microspheres were evaluated by studying the interaction between microparticles and mucin. The antigen BLSOmp31 integ...
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